vector pet 3ahistepv (New England Biolabs)
99
Structured Review
New England Biolabs
vector pet 3ahistepv
Vector Pet 3ahistepv, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 13724 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vector+pet+3ahistepv/BamHI/pm40946936-53-5-14
Average 99 stars, based on 13724 article reviews
Vector Pet 3ahistepv, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 13724 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vector+pet+3ahistepv/BamHI/pm40946936-53-5-14
Average 99 stars, based on 13724 article reviews
vector pet 3ahistepv - by Bioz Stars,
2026-10
99/100 stars
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Ligation:Article Title: Rhipicephalus microplus triosephosphate isomerase dimer interface is stabilized by a key cysteine residue. Article Snippet: The functional significance of a non-conserved cysteine residue (C86) proximal to the interfacial region of Rhipicephalus microplus triosephosphate isomerase (RmTIM) was investigated through systematic substitution with aspartic acid (C86D), lysine (C86K), and alanine (C86A) via site-directed mutagenesis.. Kinetic analyses revealed substantial perturbations in enzymatic parameters for the C86D and C86K variants, with marked alterations in maximal velocity (Vmax), substrate affinity (Km), catalytic turnover (kcat), and catalytic efficiency (kcat/Km).. Thermodynamic destabilization was universally observed across all mutants via Protein Thermal Shift assays, with reductions in melting temperature (Tm) ranging from 15.7 to 27.1 C relative to the wild-type enzyme (Wt-TIM). |